In Vivo Bioluminescence Imaging: In Vivo Imaging of Gene Expression, Tissue Transplants and Sublethal Thermal Tissue Injury
- pdf version of the abstract

In this seminar I will describe the method and application of In Vivo Bioluminescence Imaging (IBL). The concept of the use of firefly luciferase as an optical reporter gene will be discussed as well as several approaches to increase spatial resolution and sensitivity of this method. Applications of this imaging approach in studies of mouse models of tumor angiogenesis, pancreatic islet transplants and in vitro models of sublethal thermal damage inducing heat shock protein expression will be discussed.