"Colonoscopy in mice with ultrahigh resolution optical coherence tomography and laser induced fluorescence"
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Abstract: Over 15 mice models of colorectal disease exist already. In vivo colonoscopy promises to increase the information gained from mouse models while reducing costs. Optical coherence tomography (OCT) yields cross sectional structural information, such as mucosal thickness, with contrast analogous to darkfield microscopy. Recent development in high resolution endoscopic OCT allows visualization of smaller tissue structures including individual colonic crypts. Laser induced fluorescence (LIF) yields information about the development of collagen and vasculature as well as metabolic status. When spatially correlated OCT and LIF measurements are performed with the same endoscopic device in mice, significant changes in fluorescence signature have been observed over thickened adenoma relative to surrounding normal tissue. Talk starts with a description of endoscopic capability in mice and continues with some background into technologies of OCT and LIF. y.